Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Thermo Scientific Pierce Immobilized Pepsin Agarose consists of an acidic endopeptidase (pepsin) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

Pepsin is a nonspecific acidic endopeptidase produced in an inactive precursor form (pepsinogen) in the mucosal lining of the stomach of vertebrates. There are several pepsins, each with a molecular weight of 35,000 and an optimum pH of 1-3; at pH >6, the enzyme is permanently inactivated. Pepsin has broad substrate specificity. It cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Cleavage at other residues occurs with varying efficiency.

Contents

• 5mL settled gel, contains sufficient material to generate F(ab´)2 fragments from up to 20 samples containing up to 10mg of IgG