Thermo Scientific™ iodoTMTzero™ Label Reagent, 5 x 0.2 mg

Thermo Scientific™ iodoTMTzero™ Label Reagent, 5 x 0.2 mg

The Thermo Scientific iodoTMTzero Label Reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation. This tag, along with the iodoTMTsixplex reagents can enable the quantitation of protein expression changes in cell-based and tissue samples that may not be amenable to metabolic isotopic labeling strategies (e.g., SILAC). Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.

For use with: Mass Spectrometer

Features

• Specific—only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
• Irreversible—labeled proteins and peptides are not susceptible to reducing agents
• Flexible—options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
• Versatile—can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)