Thermo Scientific™ Klenow Fragment, exo– (5 U/µL), 1500 Units

Thermo Scientific™ Klenow Fragment, exo– (5 U/µL), 1500 Units

Thermo Scientific Klenow Fragment, exo-, is the large fragment of DNA polymerase I . It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site.

Features

• Lacks 3'→5' exonuclease activity
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
• Active in restriction enzyme, PCR, and RT buffers