Thermo Scientific™ LambdaXP Ligand Leakage ELISA, 1 Assay

Thermo Scientific™ LambdaXP Ligand Leakage ELISA, 1 Assay

The CaptureSelect LambdaXP Ligand Leakage ELISA detects possible presence of LambdaXP affinity ligand in the purified product after using CaptureSelect LambdaXP Affinity Matrix.

The LambdaXP affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix to give high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach from the chromatography support and co-elute with the protein. This sensitive leakage ELISA detects affinity ligand contamination with a detection limit of 1 ng/mL. The assay is designed to minimize interference and to provide accurate quantitation even in the presence of the protein of interest. The CaptureSelect LambdaXP Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.