Thermo Scientific™ Lysosome Enrichment Kit for Tissues and Cultured Cells

Thermo Scientific™ Lysosome Enrichment Kit for Tissues and Cultured Cells

The Thermo Scientific™ Lysosome Enrichment Kit for Tissue and Cultured Cells enables isolation and enrichment for intact lysosomes from crude cell and tissue extracts. The kit provides sufficient reagents for preparing 25 extracts and uses OptiPrep Cell Separation Media for the density-based separation of lysosomes from contaminating cell structures. The isolated lysosomes may be used for a number of downstream applications, including 2D/MS for proteomics research, electron microscopy, disease profiling and gene expression, signal transduction, and interaction or localization.

Features

• Efficient and easy to use—kit reagents and gradient centrifugation separate organelles from contaminating structures
• Compatible—prepare samples for downstream applications, including 2D/MS, electron microscopy, disease profiling, gene expression, signal transduction and interaction or localization studies
• Complete—kits contain sufficient material for 25 applications

Contents

• Lysosome Enrichment Reagent A: 90mL
• Lysosome Enrichment Reagent B: 90mL
• OptiPrep™ Cell Separation Media: 50mL
• BupH™ Phosphate Buffered Saline: 1 pack, results in 0.1M sodium phosphate, 0.15M sodium chloride; pH 7.2 when constituted with 500mL of ultrapure water