Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells

Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells

The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.

Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity.

Features

• Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
• Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
• Optimizable—guidelines provided for optimizing purity vs. yield with the reagent-based method
• Benchtop-easy—isolation performed in a microcentrifuge tube
• Compatible—isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis