Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
On demand
Order number :
89874
Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
The Thermo Scientific Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization. The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes. Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity.
Features
- Fast—isolate intact mitochondria from 20 million cells in approximately 40 minutes (post-cell harvest)
- Flexible—process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method
- Optimizable—guidelines provided for optimizing purity vs. yield with the reagent-based method
- Benchtop-easy—isolation performed in a microcentrifuge tube
- Compatible—isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis
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