Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Thermo Scientific™ Lysozyme Solution (50 mg/mL)

Thermo Scientific Lysozyme is an enzyme characterized by the ability to break down the bacterial cell wall to improve protein or nucleic acid extraction efficiency.

Lysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozymes (muramidases) are a family of enzymes with antimicrobial activity characterized by the ability to damage the cell wall of bacteria. The enzyme acts by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between the N-acetyl-D-glucosamine residues in chitodextrins. Although hen egg white lysozyme is most effective for the lysis of gram-positive bacteria, it also facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. The lysis of E. coli is especially improved by the addition of both lysozyme and a nucleases such as DNase I.

Features

• Bacterial cell wall lytic enzyme that improves inclusion body protein purification
• Compatible with Thermo Scientific Pierce Cell Lysis Reagents
• Clear and colorless, free of insoluble material
• pH: 4.7 ±0.2
• ≥50 units/mL at 5µg/mL