Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

The Thermo Scientific Mem-PER Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure.

The Mem-PER Plus Kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure. Integral membrane proteins having one or two membrane-spanning domains are effectively solubilized and isolated from cytosolic proteins. The kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian cells, as well as from hard and soft mammalian tissues. The Mem-PER Plus Kit has many advantages compared to our original Mem-PER Kit. The protocol is simpler, and the fractions are directly compatible with many downstream applications such as SDS-PAGE, Western Blotting, BCA, immunoprecipitation, and amine-reactive protein labeling techniques.

Features

• Extraction and isolation—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
• Cells or tissues—effective for extraction from cultured mammalian cells and mammalian tissues
• Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation and protein assays
• Fast and simple—isolation of membrane proteins in approximately one hour
• No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed

Contents

• Cell Wash Solution: 225mL
• Solubilization Buffer: 25mL
• Permeabilization Buffer: 50mL