Thermo Scientific™ Pierce™ GPCR Extraction and Stabilization Reagent

Thermo Scientific™ Pierce™ GPCR Extraction and Stabilization Reagent

Thermo Scientific GPCR Extraction and Stabilization Reagent enables efficient extraction and stabilization of G-coupled protein receptors (GPCRs) and other membrane-associated proteins from cultured mammalian cells or tissue while preserving longer-term receptor integrity.

Features

• Ease-of-use—all-in-one formulation to extract and stabilize GPCRs in one hour
• GPCR preservation—stabilizes the receptor in a detergent micelle to ensure receptor function
• Optimized—formulation and procedure provide gentle but efficient extraction of GPCRs from the plasma membrane
• Compatible—lysate is directly compatible with downstream applications including western blotting, immunoprecipitation, and receptor ligand binding assays

Contents

• Contains sufficient extraction and stabilization reagent for approximately 100 mammalian cell pellet fractions containing 1 × 10⁷ cells each or 100 tissue samples containing 50-100 mg of tissue.