Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/µL)
On demandThermo Scientific™ Micrococcal Nuclease Solution (≥100 U/µL)
Thermo Scientific Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for digestion of RNA and DNA substrates. Micrococcal Nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Features
- Enzyme digests nucleic acids (DNA and RNA)
- Effective for double-stranded, single-stranded, circular and linear nucleic acids
- Active in neutral to alkaline buffer conditions containing divalent calcium ions
- Supplied at ≥100 units/µL in 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM EDTA, 50% glycerol