Thermo Scientific™ NAb™ Protein A Plus Spin Columns, 0.2 mL

Thermo Scientific™ NAb™ Protein A Plus Spin Columns, 0.2 mL

The Thermo Scientific Protein A Plus Agarose NAb Spin Columns are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each column containing 0.2 mL of the immobilized protein resin enables quick purification of 100-1,000 µg of IgG from 25-500µL of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.

Features

• Native Protein A – immobilized Protein A is ideal for polyclonal IgG purification from human, rabbit, pig, dog or cat serum
• Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable – superior manufacturing method immobilizes Protein A by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity – this “Plus” variety of Pierce Protein A Agarose has a dense load of immobilized Protein A, providing a binding capacity greater than 34 mg human IgG/mL resin (approx. 16 to 17 mg mouse IgG/mL resin)