Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL

Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridge, 5 mL

The Thermo ScientificTM PierceTM High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.

These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTATM or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-LokTM syringe fittings or 1/16' tubing. These cartridges can be regenerated multiple times for affinity purification.

Features

• Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
• Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
• Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges