Thermo Scientific™ PDPH (3-(2-pyridyldithio)propionyl hydrazide)
On demand
Order number :
22301
Thermo Scientific™ PDPH (3-(2-pyridyldithio)propionyl hydrazide)
Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
Features
- Reactive groups: pyridyldisulfide and hydrazide
- Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
- Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
- Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
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