Thermo Scientific™ GlycoLink™ Coupling Catalyst
On demandThermo Scientific™ GlycoLink™ Coupling Catalyst
The Thermo Scientific GlycoLink Coupling Catalyst decreases reaction times and increases aldehyde-hydrazide coupling efficiency, resulting in greater than 90% coupling of glycoproteins in 4 hours.
The GlycoLink Coupling Catalyst is a two component kit composed of aniline and sodium acetate buffer. The catalyst accelerates the coupling rate of hydrazide and alkoxyamine moieties with reactive aldehydes that have been generated by periodate-oxidation of cis-diols in glycoproteins. The GlycoLink Coupling Catalyst increases the coupling efficiency of these reactions, decreasing the molar excess amounts of labeling reagents that are required for conjugation reactions. The catalyst is also useful for increasing the binding efficiency of oxidized glycoproteins or other carbohydrate-containing molecules to hydrazide resins.
Features
- Optimized—catalyzes aldehyde-hydrazide and aldehyde-alkoxyamine conjugation reaction
- Efficient—maximizes coupling efficiency and decreases required coupling reaction incubation time
- Gentle—does not interfere with protein function and is easily removed by desalting or dialysis
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