Thermo Scientific Phusion™ Plus Green PCR Master Mix, 500

Thermo Scientific Phusion™ Plus Green PCR Master Mix, 500

Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs. The master mix format simplifies reaction setup with minimal pipetting steps and retains all features of Phusion Plus DNA Polymerase. Additionally, two tracking dyes and a density reagent are pre-added to the master mix for direct loading of PCR products on gels.

Features

• High fidelity—provides >100X higher sequence accuracy than Taq polymerase
• Convenient setup—enables a universal annealing temperature of 60°C in PCR
• Enhanced specificity—hot-start mechanism reduces non-specific amplification and primer degradation
• Benchtop stability—automation enabled due to 24-hour stability of assembled reactions
• High inhibitor tolerance—works with DNA of suboptimal purity

Contents

• 2X Phusion Plus Green PCR Master Mix, 10 x 1.25 mL
• 5X Phusion GC Enhancer, 4 x 1.25 mL
• Water, 10 x 1.25 mL