Thermo Scientific™ Phire Tissue Direct PCR Master Mix, 250
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
250 Reactions
Order number :
F170S
Thermo Scientific™ Phire Tissue Direct PCR Master Mix, 250
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Features
- Direct PCR—sample is added directly to PCR reaction, therefore there is no need for time-consuming and expensive DNA purification steps
- Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
- Specially engineered Phire DNA polymerase—extremely short PCR protocol times
- Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel
Contents
- 2 x 1.25 mL Phire Tissue Direct PCR Master Mix (2X)
- 5 mL Dilution Buffer
- 3 x 100 µL DNARelease Additive
- 40 µL Control Primer Mix (25 µM each)
- 2 x 1.25 mL Water, nuclease- free
- 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)
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