Thermo Scientific™ Pierce™ Agarose ChIP Kit
On demand
Order number :
26156
Thermo Scientific™ Pierce™ Agarose ChIP Kit
The Thermo Scientific Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.
Features
- Simple and fast ChIP assay protocol (< 8 hours)
- Highly efficient isolation and lysis of nuclei
- Easy and reproducible enzymatic digestion
- Low-background and high-binding capacity Protein A/G agarose resin
- Highly specific positive controls included: RNA polymerase II antibody and GAPDH PCR primers
- Fast and reproducible spin-column format
- High-recovery DNA purification
- ChIP-validated antibodies available
Contents
Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C)
- IP Dilution/Wash Buffer (5X): 11 mL (store at 4°C)
- IP Wash Buffer 3 (5X): 4.5 mL (store at 4°C)
- IP Elution Buffer (2X): 4.5 mL (store at 4°C)
- Column Accessory Pack: 3 packs (store at room temperature or 4°C)
- Microcentrifuge Tubes (1.5mL): 75 tubes (store at room temperature or 4°C)
- DNA Clean-Up Column: 40 columns (store at room temperature or 4°C)
- DNA Column Binding Solution: 30 mL (store at room temperature or 4°C)
- DNA Column Wash Solution: 6 mL (store at room temperature or 4°C)
- pH Indicator: 0.8 mL (store at room temperature or 4°C)
- DNA Column Elution Solution: 5 mL (store at room temperature or 4°C)
- Anti-RNA Polymerase II Antibody: 25 µL (store at -20°C)
- Normal Rabbit IgG (1 mg/mL): 10 µL (store at -20°C)
- ChIP Positive Control Primers (GAPDH promoter, Human-specific): 100 µL (store at -20°C)
- Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C).
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