Thermo Scientific™ Pierce™ Agarose ChIP Kit

Thermo Scientific™ Pierce™ Agarose ChIP Kit

The Thermo Scientific Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Features

• Simple and fast ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-binding capacity Protein A/G agarose resin
• Highly specific positive controls included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format
• High-recovery DNA purification
• ChIP-validated antibodies available

Contents

Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C)

• IP Dilution/Wash Buffer (5X): 11 mL (store at 4°C)
• IP Wash Buffer 3 (5X): 4.5 mL (store at 4°C)
• IP Elution Buffer (2X): 4.5 mL (store at 4°C)
• Column Accessory Pack: 3 packs (store at room temperature or 4°C)
• Microcentrifuge Tubes (1.5mL): 75 tubes (store at room temperature or 4°C)
• DNA Clean-Up Column: 40 columns (store at room temperature or 4°C)
• DNA Column Binding Solution: 30 mL (store at room temperature or 4°C)
• DNA Column Wash Solution: 6 mL (store at room temperature or 4°C)
• pH Indicator: 0.8 mL (store at room temperature or 4°C)
• DNA Column Elution Solution: 5 mL (store at room temperature or 4°C)
• Anti-RNA Polymerase II Antibody: 25 µL (store at -20°C)
• Normal Rabbit IgG (1 mg/mL): 10 µL (store at -20°C)
• ChIP Positive Control Primers (GAPDH promoter, Human-specific): 100 µL (store at -20°C)
• Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C).