Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit

Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit

Thermo Fisher Scientific Pierce His Tag Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with His-tagged fusion proteins.

Features

• 6xHis pull-down (Product No. 21277)—purifies protein interactors of any His-tagged fusion protein
• Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
• No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
• Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
• Flexible—instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
• Less non-specific binding—Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
• Binding—Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin

Contents

Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin

• Cobalt Chelate Agarose Resin, 750 µL
• Pull-Down Lysis Buffer, 250 mL
• Imidazole Solution (4M), 5 mL
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes