Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP

Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP

The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.

The Pierce Antibody Biotinylation Kit reagents have been optimized and validated to biotin label antibodies for IP and co-IP reactions. Determining the optimal number of biotins to attach to the target molecule is one of the major challenges of biotinylation. For IP and co-IP applications, too many biotins result in reduced affinity for the target antigen, while too few biotins result in antibody leaching upon elution of the target antigen. The biotin labeling procedure in the Pierce Antibody Biotinylation Kit for IP has been developed to address this challenge.

Features

• Optimized for IP—reagents and protocols developed specifically to address antibody labeling for immunoprecipitation applications
• Easy to use—kit configuration specifically optimized to accommodate 50–200 μg of antibody in a 100 μL reaction; desalting columns are provided to remove excess biotinylation reagent after labeling
• Amine reactive—reacts with primary amines (-NH2) of antibodies
• Enhanced solubility—pegylation improves water solubility of the biotinylated antibody and prevents aggregation upon storage
• Reduced steric hindrance—longer spacer arm (29 angstroms) minimize steric hindrance when binding to avidin molecules

Contents

• EZ-Link NHS-PEG4-Biotin, 8 x 0.5 mg
• Zeba Spin Desalting Columns (7K MWCO), 8 x 0.5 mL
• 20X Phosphate Buffered Saline, 15 mL