Thermo Scientific™ Pierce™ Asp-N Protease, MS Grade

Thermo Scientific™ Pierce™ Asp-N Protease, MS Grade

Thermo Scientific Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

This Asp-N is a mass spectrometry (MS)-grade zinc metalloproteinase derived from a mutant strain of Pseudomonas fragi and requires a trace amount of zinc for activity. Asp-N can be used alone or in parallel with trypsin or other proteases to produce protein digests for peptide mapping and protein sequencing. Asp-N protease is suitable for either in-solution or in-gel digestion workflows. This Asp-N enzyme is packaged lyophilized (2 µg).

Features

• Complementary to tryptic digests—hydrolyzes proteins specifically at the amino side of aspartate and cysteic acid residues
• Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
• High specific activity—greater than 20,000 units/mg protein
• N-terminal arginine cleavage specificity—at least 90% for a complex protein sample
• Stable—provided in a lyophilized format