Thermo Scientific™ Pierce™ Cell Surface Biotinylation and Isolation Kit

Thermo Scientific™ Pierce™ Cell Surface Biotinylation and Isolation Kit

The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.

Features

• Improved performance—kit and protocol have been re-designed to improve enrichment and extraction of biotinylated plasma membrane proteins while minimizing intracellular protein contamination
• Optimized—protocol has been refreshed to reduce processing time and the number of steps, and reagent formulations optimized for improved performance
• Compatible—reagents have been verified with suspension and adherent cell lines, with additional instructions provided for MS sample preparation
• Validated applications—reagents and procedures are compatible with western blotting and MS applications

Contents

• EZ-Link Sulfo-NHS-SS-Biotin: 8 x 6 mg vials
• Lysis Buffer: 10 mL
• NeutrAvidin Agarose
• Wash Buffer: 35 mL
• Elution Buffer: 5 mL
• Column Accessory Pk: 16 spin columns, 16 caps, 16 bottom plugs, and 16 collection tubes
• Dithiothreitol (DTT), No-Weigh Format: 1 x 7.7 mg microtube
• BupH Phosphate Buffered Saline Pk: 2 Pk
• BupH Tris Buffered Saline Pk: 2 Pk