Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

The Thermo Scientific Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

Features

• Isolates cell surface proteins—reduces complexity of total cellular protein
• Efficiently recovers labeled proteins—cleavable biotin allows for nearly 100% recovery of isolated cell surface proteins
• Convenience—all reagents are provided in one kit, along with complete instructions for labeling, cell lysis and purification of cell surface membrane proteins
• Western blotting applications—proteins recovered in SDS-PAGE buffer are loaded directly onto polyacrylamide gels
• Robust system—protocol designed for diverse cell lines, including NIH 3T3, HeLa, C6 and A431