Thermo Scientific™ Pierce™ Chromatin Prep Module

Thermo Scientific™ Pierce™ Chromatin Prep Module

The Thermo Scientific™ Pierce™ Chromatin Prep Module provides a simple, fast, and reproducible method to isolate chromatin for use in assays that monitor transcription regulation through histone modification1 (epigenetics) or transcription factor-DNA binding interactions, such as chromatin immunoprecipitation (ChIP). The module contains all reagents necessary to isolate crosslinked chromatin from mammalian cells.

This Chromatin Prep Module is also included as part of our complete Agarose ChIP Kit.

Contents

Sufficient For: 30 chromatin preparations for ChIP assays

• Membrane Extraction Buffer: 10 mL
• Nuclear Extraction Buffer: 10 mL
• MNase Digestion Buffer: 5 mL
• MNase Stop Solution: 0.5 mL
• Halt Protease and Phosphatase Inhibitor Cocktail EDTA-free (100X): 4 x 90 µL
• Sodium chloride (5M): 3 mL
• Glycine solution (10X): 15 mL
• PBS (20X): 15 mL
• Micrococcal Nuclease (ChIP Grade) (10 U/µL) (store at -20°C)
• Proteinase K (20 mg/mL): 0.25 mL (store at -20°C)
• DTT, Lyophilized: 1 vial