Thermo Scientific™ Pierce™ Classic IP Kit
On demandThermo Scientific™ Pierce™ Classic IP Kit
The Thermo Scientific Pierce Classic IP Kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.
The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.
Features
- Assay consistency—spin columns (see dimensions) eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
- Fast—immunoprecipitate (IP) in less than one hour
- Mild elution conditions—recover antigen without harsh detergents or reducing agents
- Easy-to-follow instructions—purify target protein from crude lysate in four simple steps
- Suitable for most common antibody types—Protein A/G provides excellent binding for most mouse, rabbit, human and goat IgG subclasses
- Complete kit—includes lysis/wash buffer, binding and elution buffers, control agarose resin, sample loading buffer and spin columns
Contents
- Protein A/G Plus Agarose, 0.55 mL
- IP Lysis/Wash Buffer, 2 x 50 mL
- Conditioning Buffer (100X), 5 mL
- Tris-buffered Saline (20X), 25 mL
- Elution Buffer, 50 mL
- Lane Marker Sample Buffer (5X), 5 mL
- Control Agarose Resin, 2 mL
- Spin Columns and Collection Tubes
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