Thermo Scientific™ Pierce™ Classic IP Kit

Thermo Scientific™ Pierce™ Classic IP Kit

The Thermo Scientific Pierce Classic IP Kit uses high-quality Protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays with ease.

The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.

Features

• Assay consistency—spin columns (see dimensions) eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
• Fast—immunoprecipitate (IP) in less than one hour
• Mild elution conditions—recover antigen without harsh detergents or reducing agents
• Easy-to-follow instructions—purify target protein from crude lysate in four simple steps
• Suitable for most common antibody types—Protein A/G provides excellent binding for most mouse, rabbit, human and goat IgG subclasses
• Complete kit—includes lysis/wash buffer, binding and elution buffers, control agarose resin, sample loading buffer and spin columns

Contents

• Protein A/G Plus Agarose, 0.55 mL
• IP Lysis/Wash Buffer, 2 x 50 mL
• Conditioning Buffer (100X), 5 mL
• Tris-buffered Saline (20X), 25 mL
• Elution Buffer, 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Control Agarose Resin, 2 mL
• Spin Columns and Collection Tubes