Thermo Scientific™ Pierce™ Crosslink IP Kit

Thermo Scientific™ Pierce™ Crosslink IP Kit

The Thermo Scientific Pierce Crosslink IP Kit adapts the traditional IP method to include reagents and protocol for crosslinking IP antibodies to Protein A/G agarose to enable antigen immunoprecipitation without antibody contamination.

The primary benefits resulting from these features are the ability to purify target protein without contamination by the antibody and the ability to more effectively wash and separate samples from the beaded agarose resin.

Features

• Eliminates antibody contamination—antibody is irreversibly attached to the agarose beads so that co-elution of heavy and light chains with the purified protein is minimized
• Easy and efficient scalability—use only the amount of antibody needed for a single IP experiment or immobilize 100-200 µg of antibody to prepare ready-made IP affinity resin for many experiments
• Assay reliability and sample handling—Pierce Spin Columns eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
• Prepared antibody affinity resin is reusable—because the antibody is covalently immobilized and usually is not inactivated by the mild elution procedure, the resin often can be used several times
• Complete kits—package includes sufficient reagents and spin columns to perform at least 50 antibody immobilizations and IP experiments

Contents

• Protein A/G Plus Agarose, 0.55 mL
• DSS Crosslinker, 8 x 2 mg
• Coupling Buffer (20X), 25 mL
• IP Lysis/Wash Buffer, 2 x 50 mL
• Conditioning Buffer (100X), 5 mL
• Tris-Buffered Saline (20X), 25 mL
• Elution Buffer, 50 mL
• Lane Marker Sample Buffer (5X), 5 mL
• Control Agarose Resin, 2 mL
• Spin Columns and Collection Tubes