Thermo Scientific™ Pierce™ Colorimetric Protease Assay Kit

Thermo Scientific™ Pierce™ Colorimetric Protease Assay Kit

The Thermo Scientific Pierce Colorimetric Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples. This kit uses fully succinylated casein as a substrate for the assay. Hydrolysis of this substrate in the presence of protease results in the release of peptide fragments with free amino-terminal groups. These peptides are reacted with trinitrobenzene sulfonic acid (TNBSA), followed by measurement of the absorbance increase that results from the formation of yellow colored TNB-peptide adducts.

For use with equipment: Microplate Reader

Features

• Casein substrate—measure activity of any protease that cleaves casein into peptide fragments
• Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference
• Sensitive—1000 times more sensitive than assays that use unmodified forms of casein
• Simple and fast—test tube and microplate protocols are completed in less than one hour
• Homogenous—addition steps only; no separation, transfer or stop steps required
• Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest

Contents

• Succinylated Casein, 5 x 10 mg
• TNBSA Solution, 2 mL
• TPCK Trypsin Standard, 50 mg
• Borate Buffer Pack (makes 500 mL), 1 pack