Thermo Scientific™ Pierce™ Phosphoprotein Phosphate Estimation Assay Kit

Thermo Scientific™ Pierce™ Phosphoprotein Phosphate Estimation Assay Kit

The Thermo Scientific Phosphoprotein Phosphate Estimation Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

For use with: Spectrophotometer, Microplate Reader

Features

• Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr)
• Convenient—test tube and 96-well microplate protocols included and require less than 90 minutes to perform
• Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard
• Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available

Contents

Sufficient For: 500 tube assays or 1920 microplate assays

• Ammonium molybdate Solution, 25 mL
• Malachite Green Solution, 75 mL
• Phosvitin Positive Control, 1 mg
• TBS Pack (makes 500 mL), 1 pack