Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay Kit

Thermo Scientific™ Pierce™ Coomassie (Bradford) Protein Assay Kit

The Pierce Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement of total protein concentration compared to a protein standard. The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard ampules.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvettes, and microplates. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal chelating agents encountered in protein samples.

Features

• Bradford reagent—stable, ready-to-use kit of the classical Bradford assay reagent
• Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm
• Easy to use—single reagent; no working reagent preparation required
• Fast—almost immediate color development; add, mix, and read results
• Assay range—detects protein concentration in the range 1 to 1500 µg/mL
• Flexible—microplate and cuvette protocols provided and adaptable to several target working ranges

Contents

•  630 tube assays or 3800 microplate assays
•  Pierce Coomassie Assay Reagent: 950 mL
•  Albumin Standard Ampules, 2 mg/mL: 10 x 1 mL