Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.

For use with: Microcentrifuge

Features

• Improved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samples
• Reproducible—elution profiles and fractional resolution vary by less than 20%
• Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlap
• Compatible—reagents validated with a variety of complex samples, including TMT™-labeled peptides

Contents

• Reversed-phase fractionation spin columns, 12 columns
• Triethylamine (0.1%), 100 mL