Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit, 100 Reactions

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit, 100 Reactions

The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme. Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells. The stable signal output (glow) of this system provides flexibility with regard to incubation time. When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity.

For use with: Luminometer (Microplate)

Features

• Sensitive—highly sensitive detection of firefly luciferase activity
• Odorless—reagents have no foul or toxic odor, eliminating the need to work in a fume hood
• Convenient—one-step, mix-and-read protocol; no additional reagents required
• Stable—increased signal half-life and stability compared to flash assays
• Simple—does not require a luminometer with injectors
• Automation-friendly—amenable to high throughput assays

Contents

• Firefly Luc One-Step Substrate, Lyophilized (makes 1µL of 1X), store at -20°C
• Firefly Luc One-Step Assay Buffer, 11 mL, store at -20°C
• Upon receipt store kit at -20°C.