Thermo Scientific™ Pierce™ Monomeric Avidin Agarose Kit, 2 mL

Thermo Scientific™ Pierce™ Monomeric Avidin Agarose Kit, 2 mL

The Thermo Scientific Pierce Monomeric Avidin Kit enables gentle, non-denaturing affinity purification of biotinylated molecules.

Features

• Convenient kit— complete purification kit with prepacked column and buffers
• Non-denaturing—purifies biotinylated products using mild elution conditions (2 mM free biotin)
• Reusable—Monomeric Avidin Agarose can be regenerated and reused at least 10 times, with only a marginal loss in biotin binding capacity (approximately 2.5% decrease per regeneration)
• Specific—retains biotin-binding specificity and low nonspecific binding of native avidin
• Flexible—bind samples in a variety of physiological buffers and elute either with 0.1M glycine or by competition with 2 mM biotin
• Good binding capacity—greater than 1.2 mg biotinylated BSA/mL resin

Contents

• Pierce Monomeric Avidin Column, 2 mL, 1 column
• PBS Pack (makes 500 mL), 1 pack
• Biotin Binding and Elution Buffers, 200 mL
• Regeneration Buffer, 250 mL
• Column Accessories