Thermo Scientific™ Pierce™ Monomeric Avidin UltraLink™ Resin

Thermo Scientific™ Pierce™ Monomeric Avidin UltraLink™ Resin

The Thermo Scientific Pierce Monomeric Avidin UltraLink Resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobiliziation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.

The Immobilized Monomeric Avidin protein binds biotin with high specificity and moderate affinity, allowing non-biotin molecules to be washed away and then the bound biotin-labeled molecules to be competitively eluted using 2 mM biotin in phosphate buffered saline (PBS). This technique provides the gentlest elution conditions for biotinylated protein purification and avoids the contamination and other problems associated with traditional avidin and streptavidin methods. The monomeric avidin column can then be regenerated by using 0.1M glycine to strip the column of residual bound biotin without losing the ability to bind another biotinylated sample.