Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, protein A/G

Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, protein A/G

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

The Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone. The optimized kit components have been formulated to be compatible with downstream LC-MS analysis. After the immunoprecipitation procedure, the target-enriched elution fraction is ready for in-solution tryptic digestion without the need for gel purification, detergent removal, or desalting

Features

• MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS
• Sensitive—procedure successfully enriches low abundance proteins (low ng range)
• Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteins
• Robust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundance proteins (ng range) with at least 2 peptides identified per protein

Contents

• Protein A/G Magnetic Beads: 1 mL
• IP-MS Cell Lysis Buffer: 100 mL
• IP-MS Wash Buffer A: 75 mL
• IP-MS Wash Buffer B: 40 mL
• IP-MS Elution Buffer: 6 mL
• MgCl2 (1 M): 0.75 mL