Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)
On demand
Order number :
28997
Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)
Thermo Scientific Pierce TNBSA Solution is a 5% solution of trinitrobenzene sulfonic acid in methanol that reacts with primary amines (peptides or amino acids) to yield a soluble colored product, a property useful for various assay methods.
Features
- TNBSA component reacts readily with primary amino groups of amino acids in aqueous format at pH 8 to form yellow adducts
- Colored derivatives are monitored at 345nm and have extinction coefficients of 10,000 to 15,000
- Supplied as 5% solution (w/v) of TNBSA in methanol, which is easily diluted approximately 1000-fold in assay buffer for typical protein and peptide applications
- No colored derivatives are formed with secondary amino acids proline and hydroxyproline
- Applicable to solution or solid phase analysis
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