Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)

Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)

Thermo Scientific Pierce TNBSA Solution is a 5% solution of trinitrobenzene sulfonic acid in methanol that reacts with primary amines (peptides or amino acids) to yield a soluble colored product, a property useful for various assay methods.

Features

• TNBSA component reacts readily with primary amino groups of amino acids in aqueous format at pH 8 to form yellow adducts
• Colored derivatives are monitored at 345nm and have extinction coefficients of 10,000 to 15,000
• Supplied as 5% solution (w/v) of TNBSA in methanol, which is easily diluted approximately 1000-fold in assay buffer for typical protein and peptide applications
• No colored derivatives are formed with secondary amino acids proline and hydroxyproline
• Applicable to solution or solid phase analysis