Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, streptavidin
On demand
Order number :
90408
Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, streptavidin
The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.
Features
- MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS
- Sensitive—procedure successfully enriches low abundance proteins (low ng range)
- Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteins
- Reduced antibody contamination—antibody biotinylation reduces contamination in the eluate when compared to other IP methods
- Robust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundant proteins (ng range) with at least 2 peptides identified per protein
Contents
- Streptavidin Magnetic Beads, 1 mL
- IP-MS Cell Lysis Buffer, 100 mL
- IP-MS Wash Buffer A, 75 mL
- IP-MS Wash Buffer B, 40 mL
- IP-MS Elution Buffer, 6 mL
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