Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, streptavidin

Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit, streptavidin

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

Features

• MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS
• Sensitive—procedure successfully enriches low abundance proteins (low ng range)
• Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteins
• Reduced antibody contamination—antibody biotinylation reduces contamination in the eluate when compared to other IP methods
• Robust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundant proteins (ng range) with at least 2 peptides identified per protein

Contents

• Streptavidin Magnetic Beads, 1 mL
• IP-MS Cell Lysis Buffer, 100 mL
• IP-MS Wash Buffer A, 75 mL
• IP-MS Wash Buffer B, 40 mL
• IP-MS Elution Buffer, 6 mL