Thermo Scientific™ Pierce™ Protein A IgG Plus Orientation Kit, 2 mL
On demandThermo Scientific™ Pierce™ Protein A IgG Plus Orientation Kit, 2 mL
The Thermo Scientific Pierce A IgG Plus Orientation Kit uses a crosslinker to covalently attach antibody that is bound to Protein A agarose resin, thereby creating a reusable affinity column for purification of antigens.
This 'Plus' kit provides for more efficient and stable antibody immobilization than the traditional orientation method because it uses the NHS-ester crosslinker DSS (disuccinimidyl suberate) rather than the imidoester crosslinker DMP (dimethyl pimelimidate). Several milligrams of IgG can be bound and crosslinked to each 2 ml column, after which the prepared affinity column can be used multiple times for antigen purification using typical binding and elution conditions.
Features
- Contaminant-free antigen purification—provides for antigen purification without contamination (co-elution) of antibody
- Covalent, leak-resistant antibody immobilization—high-capacity binding of IgG to Protein A agarose, followed by efficient crosslinking with DSS crosslinker
- High-quality agarose resin—crosslinked 6% beaded agarose with recombinant Protein A
- Gentle binding and crosslinking conditions—coupling antibody in standard PBS
- Oriented immobilization—binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance
- Complete kit—includes all the reagents needed to immobilize the antibody
- Better than DMP—less antibody leaching than traditional orientation method with DMP crosslinker
Contents
Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column
- Recombinant Protein A Columns, 2 mL: 2 columns
- Disuccinimidyl Suberate (DSS): 2 x 13 mg
- PBS Pack (makes 500 mL): 1 pack
- Blocking Buffer: 6 mL
- IgG Elution Buffer: 15 mL
- Antibody Binding/Wash Buffer: 120 mL
- Column Accessories
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