Thermo Scientific™ Pierce™ Protein G IgG Plus Orientation Kit, 2 mL

Thermo Scientific™ Pierce™ Protein G IgG Plus Orientation Kit, 2 mL

The Thermo Scientific Pierce Protein G IgG Plus Orientation Kit uses a crosslinker to covalently attach antibody that is bound to Protein G agarose resin, thereby creating a reusable affinity column for purification of antigens.

This 'Plus' kit provides for more efficient and stable antibody immobilization than the traditional orientation method because it uses the NHS-ester crosslinker DSS (disuccinimidyl suberate) rather than the imidoester crosslinker DMP (dimethyl pimelimidate). Several milligrams of IgG can be bound and crosslinked to each 2 ml column, after which the prepared affinity column can be used multiple times for antigen purification using typical binding and elution conditions.

Features

• Contaminant-free antigen purification—provides for antigen purification without contamination (co-elution) of antibody
• Covalent, leak-resistant antibody immobilization—high-capacity binding of IgG to Protein G agarose, followed by efficient crosslinking with DSS crosslinker
• High-quality agarose resin—crosslinked 6% beaded agarose with Protein G
• Gentle binding and crosslinking conditions—coupling antibody in standard PBS
• Oriented immobilization—binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance
• Complete kit—includes all the reagents needed to immobilize the antibody
• Better than DMP—less antibody leaching than traditional orientation method with DMP crosslinker

Contents

Sufficient For: Immobilizing 1 to 6 mg of mouse IgG per column

• Protein G Columns, 2 mL: 2 columns
• Disuccinimidyl Suberate (DSS): 2 x 13 mg
• PBS Pack (makes 500 mL): 1 pack
• Blocking Buffer: 6 mL
• IgG Elution Buffer: 15 mL
• Antibody Binding/Wash Buffer: 120 mL
• Column Accessories