Thermo Scientific™ Pierce™ Protein A UltraLink™ Resin
On demandThermo Scientific™ Pierce™ Protein A UltraLink™ Resin
Thermo Scientific Pierce Protein A UltraLink Resin is an extremely durable beaded polyacrylamide support to which IgG-binding Protein A has been immobilized for use in IgG purification methods.
Protein A UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A is a popular antibody-binding protein that is especially useful for affinity purification of IgG from rabbit or human serum.
Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).
Features
- Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
- Standard capacity – loading of immobilized Protein A provides a binding capacity of approx. 16 mg human IgG per milliliter of resin
- UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications
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