Thermo Scientific™ Pierce™ Protein A Plus UltraLink™ Resin

Thermo Scientific™ Pierce™ Protein A Plus UltraLink™ Resin

Thermo Scientific Pierce Protein A Plus UltraLink Resin is an extremely durable beaded polyacrylamide support to which IgG-binding Protein A has been immobilized at high density for use in IgG purification methods.

Protein A Plus UltraLink Resin is used for high-capacity binding and purification of IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A is a popular antibody-binding protein that is especially useful for purification of IgG from rabbit or human serum.

Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The 46.7kDa-protein consists of a single polypeptide chain that is essentially devoid of carbohydrate. Native Protein A has contains four high-affinity (Ka = 10^8/mol) binding sites that are capable of interacting with the Fc region of IgG-class antibodies from selected mammalian species. IgG-binding function is optimal at pH 8.2, but efficient binding also occurs in neutral and physiological buffers (pH 7.0 to 7.6).

Features

• Immobilized Protein A – specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG
• High capacity – this “Plus” variety has a dense load of immobilized Protein A, providing a binding capacity of at least 30 mg human IgG per milliliter of resin
• UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications