Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit, 250 mL

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit, 250 mL

The Pierce Rapid Gold BCA Protein Assay Kit is a fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration. It uses the same copper-chelating technology as the well-known traditional Pierce BCA Protein Assay and provides comparable accuracy but with a 5-min, room temperature incubation and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results.

Features

• Absorbance—colorimetric assay, with optimal absorbance at 480 nm
• Uniformity—exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford)
• Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay time—5-min, room temperature reaction
• Assay range—linear working range for BSA of 20 to 2000 µg/mL

Contents

• Rapid Gold BCA Reagent A containing a proprietary copper chelator: 250 mL
• Rapid Gold BCA Reagent B: 10 mL, contains cupric sulfate
• Albumin Standard Ampules, 2 mg/mL: 10 × 1 mL ampules, contain bovine serum albumin (BSA) at 2 mg/mL in 0.9% saline and 0.05% sodium azide