Thermo Scientific™ Active Arf1 Pull-Down and Detection Kit
On demand
Order number :
16121
Thermo Scientific™ Active Arf1 Pull-Down and Detection Kit
The Thermo Scientific Pierce Active Arf1 Pull-Down and Detection Kit is a complete kit for selective enrichment and detection of GTP-bound Arf1 GTPase through specific protein interaction with the GGA3 protein-binding domain.
The Active Arf1 Pull-Down and Detection Kit includes purified GST-GGA3 protein-binding domain (PBD), glutathione agarose resin, positive and negative controls (GTPγS and GDP, respectively), lysis/binding/wash buffer, anti-Arf1 primary antibody, sample buffer, spin columns and collection tubes. The kit was validated using lysates from MDCK cells, a cell line that is known to have robust Arf1 activity.
Features
- Highly sensitive and accurate—optimized reagents, specific anti-Arf1 antibody and Western blot procedure ensure accurate controls and semi-quantitative results
- Validated—functionally tested for Arf1 detection to ensure quality and performance
- Compatible—effective with a variety of cell types from mouse, rat and human sources
Contents
- GST-GGA3-RBD Fusion Protein, 1 vial
- Anti-Arf1 Antibody, 5 units, 25 µL
- Glutathione Agarose Resin, 3 mL
- GDP (100X), 50 µL
- GTPyS (100X), 50 µL
- SDS Sample Buffer (2X), 1.5 mL
- Lysis/Binding/Wash Buffer (1X), 100 mL
- Spin Cups, 30 cups
- Collection Tubes, 90 tubes