Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis, 5 kU

Thermo Scientific™ Pierce™ Universal Nuclease for Cell Lysis, 5 kU

Thermo Scientific Pierce Universal Nuclease for Cell Lysis is ideal for a wide variety of applications where complete digestion of nucleic acids is needed when preparing cell lysates.

Pierce Universal Nuclease for Cell Lysis is a genetically engineered endonuclease from Serratia marcescens. The enzyme is produced and purified from E. coli and consists of two identical 30-kDa subunits with two critical disulfide bonds. This indiscriminate endonuclease degrades single-stranded, double-stranded, linear and circular DNA and RNA and is effective over a wide range of temperatures and pH. This enzyme has high specific activity (100-fold greater than DNase I) and increased thermal stability compared to other nucleases. Pierce Universal Nuclease is ≥99 pure enzyme, is free of any measurable protease activity and is supplied at 250U/µL. Pierce Universal Nuclease for Cell Lysis is identical in performance to Benzonase™ Nuclease (EMD Merck).

Features

• Broad spectrum—degrades all forms of DNA and RNA
• Highest-quality enzyme—nuclease is ≥99% pure, as tested by SDS-PAGE
• Robust activity—100-fold greater specific activity than DNase I
• Versatile—can be used with a wide variety of cell lysis reagents