Thermo Scientific™ Pro-Detect™ Rapid Streptag II Competitive Assay Kit

Thermo Scientific™ Pro-Detect™ Rapid Streptag II Competitive Assay Kit

The Thermo Scientific Pro-Detect Rapid Streptag II Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of Strep Tag II (sequence: PQKWSHPQFEK)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to Streptag II tag to provide a qualitative detection within ten minutes. This assay can be used to detect Streptag II-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features

• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C

Contents

• 10 Rapid Assay Strips
• Sample Diluent, 15 mL