Loader

Thermo Scientific™ pT7CFE1-NFtag Vector for Mammalian Cell-Free Protein Expression

On demand
Order number : 88865
(Ex-work price)
$ On demand

Thermo Scientific™ pT7CFE1-NFtag Vector for Mammalian Cell-Free Protein Expression


Thermo Scientific pT7CFE1-NFtag is a cloning plasmid optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins. pT7CFE1-NFtag Vector is available with single affinity Ftag tag at the N-terminus to facilitate protein purification and detection.


Features

  • EMCV IRES at the 5' UTR promotes high-level translation of mRNAs
  • MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
  • Poly A sequence in the 3' region promotes mRNA stabilization and protection from nucleases
  • T7 terminator ensures synthesis of accurate size mRNA transcripts
  • Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region


More  

There are no specifications

There are no report


You May Also Like