Thermo Scientific™ SMPT (4-succinimidyloxycarbonyl-alpha-methyl-α(2-pyridyldithio)toluene)

Thermo Scientific™ SMPT (4-succinimidyloxycarbonyl-alpha-methyl-α(2-pyridyldithio)toluene)

Thermo Scientific Pierce SMPT is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form strong (hindered) yet cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

Features

• Reactive groups: NHS ester and pyridyldithiol
• Reactive towards: amino and sulfhydryl groups
• Cleavable with disulfide reducing agents
• Water-insoluble (dissolve first in DMF or DMSO)
• Contains a hindered disulfide bond; has formed immunotoxins with improved stability
• In vitro, an SMPT conjugate was as effective as conjugates formed with SPDP and 2-IT
• Compare to other varieties of SPDP-type reagents, including pegylated forms