Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
On demand
Order number :
21650
Thermo Scientific™ Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3'-(2-pyridyldithio)propionamido)hexanoate)
Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.
Features
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
- Compare to other varieties of SPDP-type reagents, including pegylated forms
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