Thermo Scientific™ SulfoLink™ Immobilization Trial Kit, 2 mL, 1 Column

Thermo Scientific™ SulfoLink™ Immobilization Trial Kit, 2 mL, 1 Column

Thermo Scientific SulfoLink Immobilization Kit for Proteins, 2 mL is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Features

• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple protein samples in 3.5 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

Contents

• SulfoLink Column, 2 mL, 1 column
• SulfoLink Preparation Buffer, 7.5 mL
• SulfoLink Coupling Buffer, 120 mL
• Wash Solution, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• 2-Mercaptoethylamine·HCl, 6 mg
• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL
• L-Cysteine·HCl, 100 mg
• Zeba Spin Desalting Column, 5 mL, 1 column
• Column Accessories