Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate, 100 mL

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate, 100 mL

SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein detection. SuperSignal West Atto substrate provides the highest-level sensitivity and better signal-to–noise ratios compared to other commercially available high-performance horseradish peroxidase (HRP) substrates.

Features

• Sensitivity: low-femtogram to high-attogram
• Stability: 48 hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to six hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)

Contents

• Substrate Solution, 50 mL
• Stable Peroxide Solution, 50 mL