Invitrogen Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488
On demandInvitrogen Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488
To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
There are no specifications
NO related applications
There are no report
You May Also Like
Invitrogen CD262 (DR5) Monoclonal Antibody (DJR2-4 (7-8)), Unconjugated, 2 mg, eBioscience™
$ On demand
On demand
Invitrogen CD11b Monoclonal Antibody (M1/70), Biotin, 1 mg, eBioscience™
$ On demand
On demand
Invitrogen Mouse IgG2b kappa Isotype Control (eBMG2b), PE, 50 µg, eBioscience™
$ On demand
On demand
Invitrogen Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, 500 µg, eBioscience™
$ On demand
On demand
Invitrogen CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Functional Grade, 5 mg, eBioscience™
$ On demand
On demand
Invitrogen CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), Functional Grade, 500 µg, eBioscience™
$ On demand
On demand
Invitrogen CD127 Monoclonal Antibody (eBioRDR5), Super Bright 702, 25 Tests, eBioscience™
$ On demand
On demand
Invitrogen Rat IgG1 kappa Isotype Control (eBRG1), FITC, 500 µg, eBioscience™
$ On demand