Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate, 20 mL

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate, 20 mL

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Features

• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Contents

• Luminol/Enhancer: 10 mL
• Stable Peroxide Buffer: 10 mL